Wayne State University School of Medicine - Yearbook (Detroit, MI)

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A HISTOCHEMICAL STUDY OF MITOCHONDRIAL AND LYSOSOMAL ENZYMES IN THE DISEASED MYOCARDIUM This study was undertaken on portions of human myocardi- um obtained from fresh postmortem specimens. The tissue was subjected to a wide spectrum of histochemi- cal stains for mitochondrial and hydrolytic lysosomal enzymes. Mitochondrial enzymes studied were lactic, malic, succinic, NADH, and alcohol dehydrogenase. They were studied by the formazan technique. The following hydrolytic enzymes were studied: B-glucosidase, aminopeptidase, esterase, and alkaline phosphatase. A 5-bromo-4-chloro-indolyl substrate was util- ized. Hydrolysis of these substrates yields a highly insoluble indigo at the enzyme site. The myocardial diseases studied include arteriosclerotic cor- onary artery disease, hypertension, beriberi, alcoholic heart disease, brown atrophy, post-perfusion necrosis, cor pulmon- ale, acute and healed myocardial infarction and hypertrophy secondary to various causes. Abnormalities noted include: Alcoholic heart disease charac- terized by a marked decrease in zinc-dependent enzymes such as alcohol and NADH dehydrogenases, and esterase activity increase in brown atrophy of the heart. Loss and intracellular shifting of mitochondrial and lysosomal enzymes occurred in beriberi, myocardial infarct and post-perfusion necrosis. Gordon Beute Paul Wolf, M.D. 157

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EFFECTS OF DORSAL COLUMN AND SPINOCERVICO-THALAMIC PATHWAY LESIONS ON TACTILE DISCRIMINATION Ten cats, three sham-operated controls, two bilateral C-1 dorsal column lesions, two midline myelotomies and three com- bined lesions Qdorsal column, midline myelotomyj, were trained on a series of tactile discriminations in a modified Y-maze. Midline myelotomy was performed between the lower medulla and C-3 to transect cervico-thalamic pathways bilaterally. Lesions were verified histologically. Cats were trained for 30 consecutive days C20 trialsfdayj on a rough sandpaper M0- 1-1 X25 versus smooth board discrimination. Subsequently, they were trained on four different discrimination series, rough vs. extra fine C150-4f0D, line C100-ZXOD, medium C60-1f2j and extra rough C36-25. Rough C40-1-lf2Q sandpaper was always reinforced with food. Both the midline myelotomies and com- bined lesions failed to learn any series. Dorsal column lesions and controls reached 85-l0O'7b level of discrimination in all but one series. In rough vs. extra-rough discrimination, the controls reached 8070 level while the dorsal column lesions remained below 70'Z1. Results suggest that the spinocervico-thalamic pathway is necessary for tactile discrimination. The dorsal column pathway is of secondary importance except when a stimulus difference becomes small. Jerrold Weinberg Steven T. Kitai, PhD