Wayne State University School of Medicine - Yearbook (Detroit, MI)

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fx: -,L 69 C'-.ibik ll . MMM lg N--. -1 1. . 1 U X W, rnqmmtfj, Y 'J' ' timmy. ,HI I ' ' ' Illilun- ,i Lx . R I .r: I' A j, fi 1 Figif CEREBRAL DYSFUNCTION FOLLOWING HEMODILUTION PRODUCED BY THE HEART-LUNG BYPASS Cerebral dysfunction has been observed to follow hemodilution produced by the heart-lung bypass primed with :TMJ dextrose. Last summer 18 mongrel dogs were placed on the heart-lung bypass for two hours. Cerebrospinal fluid and blood samples taken before and after bypass were analysed for sodium, potassium, cal- cium, chloride, pH, PCO2, POZ, and osmolality. The proposed continuation of these investigations this summer involves the use of chronically hyponatremic dogs and radioisotopes of sodium and potassium in an attempt to determine the locations and rates of exchange of electrolytes between CSF and blood under conditions of the heart-lung bypass. john D. Fisher Clyde Wu, M.D. F236

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ANTIBIOTIC SENSITIVITY PATTERN OF URINARY BACTERIA Infection ofthe urinary tract is one of the rnost difficult thera- peutic problems in medicine today. Over the past decade numer- ous antimicrobial agents have been used in the treatment only to sec the emergence of antibiotic-resistant strains. Antimicrobial drugs have also resulted in modification in the bacterial flora commonly encountered in urinary tract infections. Recently, authors have emphasized the lethal consequences of gram- negativc bacteremia, and McCabe has demonstrated that the urinary tract is the most common site of primary infection leading to bactcriemia. ln view of the importance of this problem and the numerous antibiotics in common therapeutic use, this study was undertaken to evaluate the susceptibility of various gram-negative organisms to several antimicrobial compounds. In addition to determining the incidence of infection with various organisms, we attempted to correlate thc colony count with the presence of microscopic bactcriuria and the number of leukocytes in the urine. A total of 81 gram negative organisms were cultured from 110 urinary specimens. Fifty-nine of these had a colony count of over 50,000fml and hence were considered significant infections. E. coli was the organism most commonly cultured. Microscopic bacteruria correlated well with urinary infection but the presence of pyuria did not. Sensitivity tests demonstrated that some drugs have a predictable in vitro effect on certain organisms. Stanley Winokur N. Rachmaninoff, M.D.

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l I AGAR-GEL ELECTROPHORETIC TECHNIQUE FOR SEPARATION OF NUCLEOBASES FROM DEOXYRIBONUCLEIC ACID An agar-gel electrophoretic technique for the separation of nucleobases from purified deoxyribonucleic acid CDNAD was developed. This method is an adaptation ofa method previously employed for rihonucleic acid. DNA was extracted from a variety of microorganisms and was analyzed by two of the common techniques, thermal denaturation and buoyant density. Samples of these DNA's were then hydrolyzed with formic acid and the nucleobases spotted in high concentration on the agar gel and subjected to an EMF of 250 volts. This gave separation. The bases were then eluted from the electropherogram and quantitated by their characteristic absorption in ultra-violet light. Such a technique is important in genetic studies, where great emphasis is placed upon the DNA's molar percentage of guanine plus cytosine in determining relationships between organisms. This technique has the advantage of being simple and rapidly performed, while maintaining the accuracy ofthe more complex determinations. Perry Seese L. NI. Weiner, Ph.D. rg. .. 4 YW fs-,-.K f if 551, f ' ass, ' S 'f 2 W E tt . 'a 5 . - K i Q' N ill v Y W .- -. ., 1f'.i , WAV , ii iii: , l h,-TTAEB '- -- - . . W ,,. .... 4: . . 7 if I - E J H y 1 t D .4 .. ' 'i' x ' r 'Tis' ,af Q ig!!! .,i v can fi- , .,,.,.,,,..., my , . I V s....., ., ,EE . .H --ft sky Lg! L Y . ty -rw s - vi ..... .4 - x I Q Q it K A ' A i R' 15 1 Q 1 iv.. 1-, ' J .S .... 1 N, 1 'Su A- erik it ' ' O' i - ' 1e 'ifi - t..2:f i.' H I A