Wayne State University School of Medicine - Yearbook (Detroit, MI)

Page 150 text:

AN ELECTRON MICROSCOPIC STUDY OF PROTEIN SYNTHESIS IN THE NUCLEUS AND CORTEX OF THE DEVELOPING LENS Studies of the bovine lens soluble proteins have shown a marked and progressive decrease with age of gamma crys- tallin in the cortical lens fibers which is associated with lens fiber cell differentiation. The following study seeks to pro- vide morphological correlations which may be associated with the biochemical findings. ID A specific anti-gamma crystallin serum was obtained from rabbits immunized with purified lens antigen, and characterized using purified gamma crystallin extracts, lens extract and an anti-whole lens serum control. ZD Preliminary electron microscopic studies were carried out on the structure of cortical and nuclear lens fiber cells. 35 Preliminary correlations were obtained using an im- munofluorescent technique, and light and phase contrast microscopy. 43 Conjugation of antibodies to gamma crystallin with ferritin provides an immunoelectron microscopic method for the localization of the antigen. Larry H. Bernstein lVIaurice H. Bernstein, Ph.D Harry Maisel, M.D. 148

Page 149 text:

THE RELATION OF THE WHITE BLOOD CELLS TO A THROMBUS Thrombus formation was induced in a peripheral vein of dog, and after a designated period of time, the vessel was emoved. Material clinging to the area of the vessel which ad been traumatized to initiate thrombogenesis was treaked across slides, stained with Leishmans differential 'tain, and examined under the microscope. Differential hite cell counts were done on the material. The purpose f the project was to demonstrate whether the various white 'ell types are randomly entrapped in the thrombatic mate- ial, or whether there is a directed migration of various hite cell types into the thrombatic material at different ntervals of time. The latter situation was found to be the ne occurring in our experimental animals. Linda Louise Stodulski Raymond Henry, Ph.D.

Page 151 text:

'wsnillllllllil i fl 'l11'!!1!'.l..i:i,s it I lltllir 1 l ll 'lllgl WSE ll , ' gwglllrzl in .limlllliiti . l1LH..lE'1iIvwm1lll1 e I , llllllllll if ,l -. . filth: 1 W,,,,c - 'N , ll ll l E E uname sm 1 'll ll' f 1 Hull' Inu ,sf .k FX x5 ,ii .. THE ONTOGENY OF SOLUBLE LENS PROTEINS The soluble lens proteins were studied using biochemical electrophoretic and immunological techniques. The problem of lens differentiation was studied in several parts as follows: lj Qualitative and quantitative changes in crystallin composition during development. 25 Nuclear and cortical differences in protein composi- tion during development. 3D Embryonic proteins Cwith gamma crystallin mobilityl were identified by starch gel electrophoresis in lenses of several species. The gamma crystallins of the beef lens were eluted from starch gel and studied immunologically. 45 The characterization of protein composition in the lens epithelial cell. , 5D Changes in LDH activity during lens aging, and nu- clear and cortical differences. 65 LDI-I isozyme activity in the lens epithelial cell. Larry H. Bernstein Harry Maisel, M.D. 149