Wayne State University School of Medicine - Yearbook (Detroit, MI)

Page 149 text:

THE RELATION OF THE WHITE BLOOD CELLS TO A THROMBUS Thrombus formation was induced in a peripheral vein of dog, and after a designated period of time, the vessel was emoved. Material clinging to the area of the vessel which ad been traumatized to initiate thrombogenesis was treaked across slides, stained with Leishmans differential 'tain, and examined under the microscope. Differential hite cell counts were done on the material. The purpose f the project was to demonstrate whether the various white 'ell types are randomly entrapped in the thrombatic mate- ial, or whether there is a directed migration of various hite cell types into the thrombatic material at different ntervals of time. The latter situation was found to be the ne occurring in our experimental animals. Linda Louise Stodulski Raymond Henry, Ph.D.

Page 148 text:

IMMUNOGENIC CHA RACTERISTICS OF BOVINE PANCREATIC RIBONUCLEASE AS DETERMINED BY PASSIVE CUT ANEOUS ANAPHYLAXIS When the immune reaction occurs in the skin h . , t ere results an increase in the permeability of the capillaries. This reaction can be visualized if a dye is injected intra- venously along with thc antigen several hours after the intradermal injection of antibody. Passive cutaneous anaphylaxis CP.C.A.l was utilized t study the immunologic properties of the enzyme ribonu clease. It was shown that the extent of P.C.A. varies directl with the quantity of antibody injected intradermally. Another finding was that certain divalent polypeptide frag ments of the intact antigen were sufficient to elicit P.C.A while particular univalent polypeptides could inhibit th combination of the antigen with its antibody. This inhibi tion was effective only if the polypeptide was injecte immediately prior to challenge with the antigen. This in hibition could be prolonged if the polypeptide was ester ifiedg thereby forming a compound of linking covalentl with the antigen-combining sites on the antibody molecule Lawrence F. Gaynor Ray K. Brown, M.D., Ph.D.

Page 150 text:

AN ELECTRON MICROSCOPIC STUDY OF PROTEIN SYNTHESIS IN THE NUCLEUS AND CORTEX OF THE DEVELOPING LENS Studies of the bovine lens soluble proteins have shown a marked and progressive decrease with age of gamma crys- tallin in the cortical lens fibers which is associated with lens fiber cell differentiation. The following study seeks to pro- vide morphological correlations which may be associated with the biochemical findings. ID A specific anti-gamma crystallin serum was obtained from rabbits immunized with purified lens antigen, and characterized using purified gamma crystallin extracts, lens extract and an anti-whole lens serum control. ZD Preliminary electron microscopic studies were carried out on the structure of cortical and nuclear lens fiber cells. 35 Preliminary correlations were obtained using an im- munofluorescent technique, and light and phase contrast microscopy. 43 Conjugation of antibodies to gamma crystallin with ferritin provides an immunoelectron microscopic method for the localization of the antigen. Larry H. Bernstein lVIaurice H. Bernstein, Ph.D Harry Maisel, M.D. 148