University at Buffalo School of Medicine - Yearbook (Buffalo, NY)

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-ff ,F- I p ' ' l 'L vi I . Quik I a A ' 1 l I A- I ' 'll' in . I .---- G , . u . Carl Gerard's research proiecl was car- rled oul in +he Biophysics deparimenl' under lhe lulelage of Dr. Sidney Shulman. His sludy was confined lo lhe purificalion of human plasminogen, a proenzyme o'F ihe fibrinolyfic or clol-lysing prolein plasmin. The densil -gradienl' ulfracenlrifugafion of moderaleaf pure malerial was +he ap- proach used. This procedure requires a densily gradienl' of carefully layered su- crose solulions of decreasing concenlra- lions upon which lhe prolein sample is layered. The preparafion is subsequenlly ceniriluged for several hours al high speed lapproximalely l50,000 'limes gravilyl lo Separale l'he sample proleins according +o Their molecular weighls and sedimenfa- hon ra'l'es. The preparalion is 'lhen frac- 'lionaied +o sample +he prolein al' various Cleplhs in fhe gradienl. The samples are analyzed 'For profein concenlraiion and as- Sayed 'For polenlial proleolylic acl'ivi'Iy. Those samples showing marked improve- menl' were examined in lhe ullracenlriluge 'lor 'lheir physical homogeneily. STUDIES IN THE IDENTITY OF THE AN- TIGEN IN THE THYROID CELLS REAC- TIVE WITH AUTOANTIBODIES IN HU- MAN SERA FROM THYROIDITIS PA- TIENTS - Joseph Gerbasi The sera of palienls wilh chronic 'l'hy- roidilis have been shown lo conlain anli- bodies which reacl' wilh componenis of normal human 'lhyroid glands. These an'li- bodies can be shown +o reacl wilh lhe col- loid and 'follicular epilhelium by immuno- fluorescenl' slaining. One of lhe anligens presenl in lhe colloid has been shown lo be fhyroglobulin. Wilh lhe use of lhe immuno- fluorescenl' 'lechnique and specific enzy- ma'I'ic digeslions, sludies were made l'o idenfily lhe anligenlsl presenl' in lhe fol- licular epilhelium. gs? THE BEHAVIORAL AND EEG CHANGES INDUCED IN RATS BY THE INTRA- VENTRICULAR ADMINISTRATION OF URACIL METABOLITES AND ANA- LOGUES - Marla Pefer Heilbrun. Following fhe adminislralion of 6-azaur- acil, an anli-neoplaslic ageni, lo cancer palienfs, neurological sympioms and EEG changes became apparenl. In order lo siudy +he mechanism of neuroloxicify, 6- azauracil and ofher uracil analogues were adminislered +o rafs by in'ec+ion inlo lhe lhird venfricle. Slrilring behavioral and EEG changes occurred characlerized by de- pressed behavior and large amplifude slow waves on ihe EEG. The relalion of 'lhese changes impaired RNA and roiein syn- lhesis in neuronal and glial iiinclion was considered as a possible mechanism of drug aclion. if i N The clerlrships offered by lhe American Cancer Sociely, lhrough Harold Solomon, D.D.S., af Roswell Parl: Memorial Hospilal, made if possible 'For lhree denlal sludenls 'lo observe cancer and allied diseases. One sludeni' is assigned lo Memorial Hospi+al in New Yorlc Cily and fwo are assigned 'lo Roswell Parlr in. Buffalo. These clerlcships allow lhe sludenl lo observe a wide variely of cancerous oral lesions and lo allow lhe sludenl 'lo see cancer in all of ils sfages so l'ha+ he will be properly equipped lo malce an early diagnosis in his praclice and help combal' lhis dread disease by one of 'I'he besf melhods lmown loday, early delecfion. Richard Overgaard. Ross Wyman, and Howard Roclcolif gall Denl. '62l were awarded lhese 'fellowships during 'lhe summer of '6I. Mr. Roclco cenlered his sludies on 'lhe Plummer-Vinson Syndrome, while Messrs. Overgaard and Wyman sludied ameloblaslomas of lhe head and neck regions. The Plummer-Vinson Syndrome is lhoughl' especially signiiicanl as a pre-dis osing cause of oral and pharyngeal malignancy. Mr. RoclcoFl's findings were published in H7 +he December 'bl Issue of The Denial Sfudenis' Magazine.

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The following fopics were sfudied by Ronald Dozorefz in associafion wifh Dr. Julian Ambrus af fhe Roswell Park Me- morial lnsfifufe. The value of fibrinolysin fherapy for peri- pheral vascular fhrombosis and hyaline membrane disease. A double-blind sfudy evaluafing combi- nafion drug fherapy analgesia in cancer pafienfs. The anfi-cancer value of AB-l32, one of a series of dual anfagonisfs. ,599 PHARMACOLOGICAL STUDY - Paul Loree Mr. Wiggin, sfudying under Dr. House- mann of fhe Pharmacology Deparfrnenf, worked ,wifh immafure rafs in an efforf fo produce fhe hypofhyroid sfafe by iniecfing 300 microcuries of I131 infraperifoneally. He 'Found fhaf in his lof of l00 rafs fhaf 'lhe basal mefabolism rafe, body weighf, and hearl' rafe significanfly decreased af- fer fwo and one-half weeks, and fhaf fhis mefhod seemed superior fo surgery or fhe use of anfifhyroid drugs. '27 l PURIFICATION OF THYROID PROTEINS BY COLUMN CHROMOTOGRAPHY Joseph Armenia By sfepwise elufion column chromof09' raphy on diefhylaminoefhyl cellulose fhyr0' globulin was purified fo approximafely 992 ulfracenfrifugal homogeneify from 6 crude saline exfracf of fhyroid gland. L TISSUE IMMUNIZATION - Roberl' Genco. Tissue immunizafion sfudies were conducfecl in fhe lab- The long acfing coronary vasodilafor enfaeryfhrifal fefranifrafe produces a sfafisfically signifiicanf decrease in The nifroglycerine requiremenfs of pafienfs wifh an- gina pecforis. Addifion of franquilizers or phenobarbifal fails fo enhance fhe acfion of fhe drug alone. Six hundred pafienfs freafed in fhe psychiafric service of E. J. Meyer Memorial Hospifal wifh Promazine were found fo have neifher physical nor chemical evidence of he afofoxicify or bone marrow cle ression. These side eflzcfs have been reporfed for chloor romazine. Trans- ienf hypofension was fhe only side eigecl' presenf wifh Promazine as used on fhis service according fo fhe manu- facl'urer's suggesfed dosage schedule. II6 orafories of Dr. Ernesf Beufuer in fhe Deparfmenf of Bac- feriology and Immunology. Animal immunizafion wifh salivar glands was fhe basic area of sfudy. lf was found fhal' afler immunizafion wifh submaxillary glands, rab- bifs may produce anfibodies fhaf acf in vifro wifh some of fheir own salivary glands. This fype of anfibody is referred fo as an au'l'oan'l'ibody. The formafion of aufoanfibodies is a remarkable phe- nomena and has been shown fo be associafed wifh sev- eral human diseases, some of which are of proven aufo- immune efiology. These experimenfs were direcfed af elucidafing Siorgren's Syndrome, a human disease char- acferized by lack of salivary and lacrimal secrefions.

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fDr. william jiarveg