Chicago School of Medicine and Surgery - Medicos Yearbook (Chicago, IL)

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l 1 LITERATURE In our first examinations we put through three different tests with all serums. In the first the serum were activated and guinea-pig serum added for complement. In the second the serums were not inactivated, but fresh guinea-pig serum was also added. In the third the serums were used in active state, but no additional complement was used. 1 We found that the first. series would work out satisfactorily, the seccnd series was erratic on acccunt of anticomplementary substances, above referred to, and the third series showed little or no hencolysis in practically all tubes. . Fresh guinea-pig serum was used as complement and rabbit antisheep arrbcceptcr and sheep's corpuscles were employeed as the hemolytic system. Emory speaks of non-tuberculous serunvs anchor- ing complement in the presence of bacilli emulsion, the chief difference between ncn-tuberculous and tuberculous serums being in the latter absorbing complement in shorter time. We are not able to conhrrn this observation. The protocol of a reaction would read as follows: T. B.'Baeilli Patient's Guinea- 4 Anti- Sheep Hemolysis? , Emulsion Serum Pig ' Sheep Corpuscles, 5 'Z ' 3' V - Serum Amboceptor Suspension all 0.05 0.1 0.1 . 0.002 1 N0 'fl 0.05 0.2 0.1 0.002 1 No ' 0.025 P 0.1 0.1 0.002 1 No. , , 0.02S 0.2 0.1 0.002 1 No 0.05 0.1 0.002 1 Yes iii ,0.02S- 0.1 0.002 1 Yes Q ..... 0.4 o.1 0.002 I Yes 5 l i ll The quantities of antigen and amboceptor employed are, of course, variable and depend on their 'ffl strength. It is necessary to titrate the antigen and amboceptor before each test. Therefore, the gl above is sim lf a dia rammatic re resentation of the amounts used. .1 P 3 S P The amboceptor should be used in twice the strength sufficient to hemolyze the corpuscles in from 1 fifteen to twent minutes. X 1 Y In making the reaction, the tubes must be closely watched after being flnally placed in an incubator. A reading should be made as soon as the controls on the hemolytic system, tuberculin and a few known controls hemolyze. The degree of inhibition of hemolysis varies from 1 to 4. Eventually in practically all instances the hemolysis will be complete, but the delay in hemolysis with tuberculous serums is striking in contrast to the promptness with which the controls hemolyze. We ,examined the serums of 100 cases. Sixty-five of these were tuberculous or suspects. The remainder were control cases, consisting chiefiy of acute infections, as pneumonia, erysipelas, typhoid fever,,catarrhal jaundice, infiuenza, etc. The patients from whom the specimens of blood were obtained were.i-n the tuberculous department and medical wards of the Cook County Hospital and in my Willard Hospital clinic. ' Of the tuberculous cases, 72 per cent gave a positive reaction with human tubercle emulsion as antigen and 23 per cent gave a positive result with bovine emulsion as antigen, on only one instance did we get a reaction with bovine emulsion and not with human. Of the thirty-five controls, three gave a reaction with human tubercle bacilli emulsion. One of them was a pneumonia, another an influenza and the third case was one of rheumatism. One control gave a reaction with the bovine emulsion. In one case of cancer and one of chronic nephritis, the heated serums as Well as the inactivated serums with antigen inhibited hemolysis to some extent. This is an experience, above referred to, that we frequently observed with active serums in general and not infrequently in cancer serums which had been inactivated. Q In view of the first experiences with the lues reaction, we have every reason to feel encouraged and to believe that this reaction will 'be brought to a high degree of efficiency in the diagnosis of tuberculosis. Page I80 l 1

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Page 182 LITERATURE when When the days of school are over, When with our books We are done, When the Springtime brings the clover, And the Graduations come. When no longer We meet for classwork, When from reciting We are free, When no regrets for exams in our minds, And our teachers We no longer see. When the school spirit no longer cheers us, When the glamor of school is goneg When our classmates no longer greet us, And We meet none the Whole day long. When this is our realizaticn. When our destinies are far and Wide, When We've severed our school relations, And companionship found and tried. When alone, Will you get lonely, . Will. school memories be the same, Will you Wish then for the privelege, only To clasp a classmate's hand again. ' Will you long then for the present, Wlith its friendly congenial air, lurk Will the memories be as pleasant, i As now prevails most everywhere. Will you long then just a little, For your classmates, pal, and friend, For the privileges that are ours now And the joys therewith attend. Whenisuch, dear classmates, is your subject, Then you surely can depend, That you'll find a mutual interest- That friendship of friend for friend. W. R. C. ,....,.., .-. ..,.,,... ..,.,,.,.,.,,. -.-,.,-...,..,:g......-,..,...,.,,-...- V. .1 .,.,. -.v ,ua .g.,......,-.. .-.. ,..i -...W Va. .,..,, .,-,.,,.,,,,,,,,,f,V.,,,, , ,,-. ...ng-ff---.